| Series Code |
Age |
Experimental Conditions |
Format |
Series Length |
| K15 |
49-50d |
Perf w/ 2% Para, 2.5% glut, OSO4, T.A., U.A. enBloc |
|
55 |
| K16 |
49-50d |
Perf w/ 2% Para, 2.5% glut, OSO4, T.A., U.A. enBloc |
|
62 |
| K17 |
38-39d |
Perf w/ 2% Para, 2.5% glut in NaCacod buffer, Glycerol
U.A., OSO4, OSO4 & KFeCn |
|
56 |
| K18 |
38-39d |
Perf w/ 2% Para, 2.5% glut in NaCacod buffer, Glycerol
U.A., OSO4, OSO4 & KFeCn |
5X8, 8X10, Digital* |
71 |
| K21 |
77d |
Perf w/ 2% Para, 2.5% glut-6 different preps: 1) 1% OSO4
w/ U.A. enBloc, 2) 1% OSO4 without U.A. enBloc, 3) KFeCN with U.A. enBloc,
4) KFeCN without U.A. enBloc, 5) KFeCN, 1% OSO4 with U.A. enbloc, 6) KFeCN,
1% OSO4 without U.A. enBloc |
5X8, 8X10, Digital* |
68 |
| K22 |
77d |
Perf w/ 2% Para, 2.5% glut-6 different preps: 1) 1% OSO4
w/ U.A. enBloc, 2) 1% OSO4 without U.A. enBloc, 3) KFeCN with U.A. enBloc,
4) KFeCN without U.A. enBloc, 5) KFeCN, 1% OSO4 with U.A. enbloc, 6) KFeCN,
1% OSO4 without U.A. enBloc |
8X10 |
79 |
| K24 |
77d |
Perf w/ 2% Para, 2.5% glut-6 different preps: 1) 1% OSO4
w/ U.A. enBloc, 2) 1% OSO4 without U.A. enBloc, 3) KFeCN with U.A. enBloc,
4) KFeCN without U.A. enBloc, 5) KFeCN, 1% OSO4 with U.A. enbloc, 6) KFeCN,
1% OSO4 without U.A. enBloc |
8X10, Digital |
89 |
| K31 |
7d |
Perf w/ 2% Para, 2.5% glut in .1M Nacacod buffer, 5 preps:
1) OSO4, U.A. enbloc 2) OSO4, U.A. enbloc 3) KFeCN-OSO4, OSO4, T.A., U.A.
enbloc 4) KFeCN-OSO4, OSO4, T.A., U.A. enbloc 5) KFeCN-OSO4, OSO4, U.A.
enbloc |
|
| K32 |
15d |
Perf w/ 2% Para, 2.5% glut in .1M NaCacod buffer; 6 preps:
1) OSO4, T.A., U.A. enbloc 2) OSO4, U.A. enbloc 3) KFeCN-OSO4, OSO4, U.A.
enbloc 4) KFeCN-OSO4, OSO4, T.A., U.A. enbloc 5) KFeCN-OSO4, OSO4, U.A.
enbloc 6) KFeCN-OSO4, OSO4, buffer overnight, U.A. enbloc |
8X10 |
37 |
| K33 |
77d |
Perf w/ 2% Para, 2.5% glut-6 different preps: 1) 1% OSO4
w/ U.A. enBloc, 2) 1% OSO4 without U.A. enBloc, 3) KFeCN with U.A. enBloc,
4) KFeCN without U.A. enBloc, 5) KFeCN, 1% OSO4 with U.A. enbloc, 6) KFeCN,
1% OSO4 without U.A. enBloc |
8X10 |
27 |
| K34 |
77d |
Perf w/ 2% Para, 2.5% glut-6 different preps: 1) 1% OSO4
w/ U.A. enBloc, 2) 1% OSO4 without U.A. enBloc, 3) KFeCN with U.A. enBloc,
4) KFeCN without U.A. enBloc, 5) KFeCN, 1% OSO4 with U.A. enbloc, 6) KFeCN,
1% OSO4 without U.A. enBloc |
11X14 |
33 |
| K35 |
15d |
Perf w/ 2% Para, 2.5% glut in .1M NaCacod buffer; 6 preps:
1) OSO4, T.A., U.A. enbloc 2) OSO4, U.A. enbloc 3) KFeCN-OSO4, OSO4, U.A.
enbloc 4) KFeCN-OSO4, OSO4, T.A., U.A. enbloc 5) KFeCN-OSO4, OSO4, U.A.
enbloc 6) KFeCN-OSO4, OSO4, buffer overnight, U.A. enbloc |
11X14, Digital |
35 |
| K36 |
77d |
Perf w/ 2% Para, 2.5% glut-6 different preps: 1) 1% OSO4
w/ U.A. enBloc, 2) 1% OSO4 without U.A. enBloc, 3) KFeCN with U.A. enBloc,
4) KFeCN without U.A. enBloc, 5) KFeCN, 1% OSO4 with U.A. enbloc, 6) KFeCN,
1% OSO4 without U.A. enBloc |
11X14 |
68 |
| K37 |
38-39d |
Perf w/ 2% Para, 2.5% glut in NaCacod buffer, Glycerol
U.A., OSO4, OSO4 & KFeCn |
11X14 |
54 |
| K38 |
38-39d |
Perf w/ 2% Para, 2.5% glut in NaCacod buffer, Glycerol
U.A., OSO4, OSO4 & KFeCn |
11X14 |
46 |
| K39 |
15d |
Perf w/ 2% Para, 2.5% glut in .1M NaCacod, 2 preps: 1)OSO4,
U.A. enbloc 2) KFeCN-OSO4, OSO4, buffer, U.A. enbloc |
11X14 |
39 |
| K40 |
15d |
Perf w/ 2% Para, 2.5% glut in .1M NaCacod, 2 preps: 1)OSO4,
U.A. enbloc 2) KFeCN-OSO4, OSO4, buffer, U.A. enbloc |
11X14 |
39 |
| BFPD |
22d |
Perf w/ 2% Para, 6% glut in .1M NaCacod buffer. Tissue
then MW processed in KFeCN-OSO4, OSO4, U.A. enbloc; tissue then infiltrated
in EPON/Spurr's at room temperature and cured in 60 degrees C oven |
8X10, Digital* |
112 |
| CTFL |
68d |
Perf w/ 6% glut, 2% Para in .1M NaCacod buffer. Tissue
was MW processed in KFeCN-OSO4, OSO4, U.A. enbloc; tissue infiltrated in
EPON/Spurrs at room temp and cured in 60 degrees C oven |
8X10 |
98 |
| DHDT |
21d |
Slice MW-fixed in 2% Para, 6% glut in 0.1M Cacod buffer.
Tissue then MW processed in KFeCN-OSO4, OSO4, U.A. enbloc; tissue infiltrated
in EPON/Spurr's at room temp and cured in 60 degrees C oven |
8X10, Digital* |
112 |
| FGDS |
66d |
Slice MW-fixed in 2% Para, 6% glut in 0.1M Cacod buffer.
Tissue then MW processed in KFeCN-OSO4, OSO4, U.A. enbloc; tissue infiltrated
in EPON/Spurr's at room temp and cured in 60 degrees C oven |
8X10 |
121 |
| GSNX |
22d |
Slice MW-fixed in 2% Para, 6% glut in 0.1M Cacod buffer.
Tissue then MW processed in KFeCN-OSO4, OSO4, U.A. enbloc; tissue infiltrated
in EPON/Spurr's at room temp and cured in 60 degrees C oven |
8X10 |
96 |
| JYNQ |
21d |
Perf w/ 2% Para 6% glut in .1M NaCacod buffer. Tissue
then MW processed in KFeCN-OSO4, OSO4, U.A. enbloc; tissue infiltrated
in EPON/Spurr's at room temp. and cured at 60 degrees C |
8X10, Digital |
134 |
| KPRT |
SKDiIO7 |
Slice immersion fixed-tissue then microwaved processed
in KFeCN-OSO4, OSO4, U.A. enbloc; tissue infiltration in EPON/Spurr's at
room temp and cured in 60 degrees C oven |
8X10 |
110 |
| LMTN |
66d |
Slice MW-fixed in 2% Para, 6% glut in 0.1M Cacod buffer.
Tissue then MW processed in KFeCN-OSO4, OSO4, U.A. enbloc; tissue infiltrated
in EPON/Spurr's at room temp and cured in 60 degrees C oven |
8X10 |
107 |
| MXWT |
SKDiIO4 |
Slice MW-fixed in 2% Para, 6% glut in 0.1M Cacod buffer.
Tissue was MW processed, KFeCN-OSO4, OSO4, U.A. enbloc, tissue then infiltrated
in EPON at room temp and cured in 60 degrees C oven |
8X10 |
109 |
| RFBL |
SKDiIO5 |
Slice MW-fixed in 2% Para, 6% glut in 0.1M Cacod buffer.
Tissue was MW processed, KFeCN-OSO4, OSO4, U.A. enbloc, tissue then infiltrated
in EPON at room temp and cured in 60 degrees C oven |
8X10 |
103 |
| WRPV |
68d |
Perf w/ 6% glut, 2% Para in .1M NaCacod buffer. Tissue
then MW processed in KFeCN-OSO4, OSO4, U.A. enbloc. Tissue then infiltrated
in EPON/Spurr's at room temp and cured at 60 degrees C in oven. |
8X10 |
106 |
| ZNQB |
65d |
Slice MW-fixed in 2% Para, 6% glut in 0.1M Cacod buffer.
Tissue then MW processed in KFeCN-OSO4, OSO4, U.A. enbloc; tissue infiltrated
in EPON/Spurr's at room temp and cured in 60 degrees C oven |
8X10 |
109 |
| ZYGR |
65d |
Slice MW-fixed in 2% Para, 6% glut in 0.1M Cacod buffer.
Tissue then MW processed in KFeCN-OSO4, OSO4, U.A. enbloc; tissue infiltrated
in EPON/Spurr's at room temp and cured in 60 degrees C oven |
8X10 |
116 |
| R43a |
6d |
Perf w/ 2% Para, 2.5% glut in .1M Nacacod buffer. Then
tissue was MW processed in KFeCN-OSO4, OSO4, U.A. enbloc, followed by EPON/Spurr's
infiltration & Cured in MW |
8X10 |
|
| R43b |
6d |
Perf w/ 2% Para, 2.5% glut in .1M Nacacod buffer. Then
tissue was MW processed in KFeCN-OSO4, OSO4, U.A. enbloc, followed by EPON/Spurr's
infiltration & Cured in MW |
8X10 |
|
| R44a |
6d |
Perf w/ 2% Para, 2.5% glut in .1M NaCacod buffer. Then
tissue was MW processed in KFeCN-OSO4, OSO4, U.A. enbloc, followed by EPON/Spurr's
infiltration & Cured in MW |
8X10 |
|
| R44b |
6d |
Perf w/ 2% Para, 2.5% glut in .1M NaCacod buffer. Then
tissue was MW processed in KFeCN-OSO4, OSO4, U.A. enbloc, followed by EPON/Spurr's
infiltration & Cured in MW |
8X10 |
|
| R45a |
12d |
Perf w/ 2% Para, 2.5% glut in .1M NaCacod buffer. Then
tissue was MW processed in KFeCN-OSO4, OSO4, U.A. enbloc, followed by EPON/Spurr's
in filtration & cured in MW |
8X10 |
|
| R45b |
12d |
Perf w/ 2% Para, 2.5% glut in .1M NaCacod buffer. Then
tissue was MW processed in KFeCN-OSO4, OSO4, U.A. enbloc, followed by EPON/Spurr's
in filtration & cured in MW |
8X10 |
|
| R47b |
12d |
Perf w/ 2% Para, 2.5% glut in .1M NaCacod buffer. Then
tissue was MW processed in KFeCN-OSO4, OSO4, U.A. enbloc, followed by EPON/Spurr's
infiltration & cured in MW |
8X10 |
|
| R47c |
12d |
Perf w/ 2% Para, 2.5% glut in .1M NaCacod buffer. Then
tissue was MW processed in KFeCN-OSO4, OSO4, U.A. enbloc, followed by EPON/Spurr's
infiltration & cured in MW |
8X10 |
|
| R48a |
4d |
Perf w/ 2% Para, 2.5% glut in .1M NaCacod buffer. Then
tissue was MW processed in KFeCN-OSO4, OSO4, U.A. enbloc, followed by EPON/Spurr's
infiltration & Cured in MW |
8X10 |
|
| R48b |
4d |
Perf w/ 2% Para, 2.5% glut in .1M NaCacod buffer. Then
tissue was MW processed in KFeCN-OSO4, OSO4, U.A. enbloc, followed by EPON/Spurr's
infiltration & Cured in MW |
8X10, Digital |
|
| R52a |
1d |
Perf w/ 2% Para, 2.5% glut in .1M NaCacod buffer. Then
tissue was microwave processed in KFeCn-OSO4, OSO4, U.A. enbloc, followed
by EPON/Spurr's infiltration and cured in 60 degrees C oven instead of
MW, due to technical error |
8X10 |
|
| R52b |
1d |
Perf w/ 2% Para, 2.5% glut in .1M NaCacod buffer. Then
tissue was microwave processed in KFeCn-OSO4, OSO4, U.A. enbloc, followed
by EPON/Spurr's infiltration and cured in 60 degrees C oven instead of
MW, due to technical error |
8X10 |
|
| R53 |
1d |
Perf w/ 2% Para, 2.5% EM glut in .1M NaCacod buffer.
Then tissue was MW processed in KFeCN-OSO4, OSO4, U.A. enbloc, followed
by EPON/Spurrs infiltration and cured in 60 degress C oven instead of MW,
due to technical error |
8X10 |
|
| R55 |
1d |
Perf w/ 2% Para, 2.5% glut in .1M NaCacod buffer. Then
tissue was MW processed in KFeCN-OSO4, OSO4, U.A. enbloc, followed by EPON/Spurrs
infiltration and cured in 60 degrees C oven, instead of MW, due to technical
error |
8X10 |
|
| R60a |
4d |
Perf w/ 2% Para, 2.5% glut in .1M NaCacod buffer. Then
tissue was MW processed in KFeCN-OSO4, OSO4, U.A. enbloc, EPON/Spurr's
infiltrated and cured in 60 degrees C oven due to a technical error |
8X10 |
|
| R60b |
4d |
Perf w/ 2% Para, 2.5% glut in .1M NaCacod buffer. Then
tissue was MW processed in KFeCN-OSO4, OSO4, U.A. enbloc, EPON/Spurr's
infiltrated and cured in 60 degrees C oven due to a technical error |
8X10, Digital |
|
